Journal:

Article Title: Constitutive Expression of a Chromosomal Class A (BJM Group 2) ?-Lactamase in Xanthomonas campestris

doi: 10.1128/AAC.48.1.209-215.2004

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Antibiotics were added when necessary: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Characteristic(s) Source or reference E. coli DH5α endA1 hsdR17 (r k − m k + ) supE44 thi-1 recA gyrA relA1 φ80d lacZ ΔM15 Δ( lacZYA - argF ) Bethesda Research Laboratories ATCC 25922 Reference strain for MIC tests American Type Culture Collection E. coli (pFY13-9BSRX) DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and bla XCC-1 genes, Km r /Ap s This study E. coli (pBX) DH5α with plasmid pBX carrying the bla XCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tc r /Ap s This study E. coli (pRX) DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tc r /Ap s This study X. campestris pv. campestris Xc2 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection a Xc6 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc85 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc17 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11A Spontaneous avirulent mutant from Xc11, Ap r Yang and Tseng ( 33 ) Xc11bla::Tc Xc11 bla XCC-1 mutant constructed by inserting a Tc r cartridge into the Sma I site of the bla XCC-1 gene, Tc r /Ap s Weng et al. ( 29 ) Xc11RM Xc11 ampR1 mutant constructed by inserting a Tc r cartridge into the Sal I site of the ampR1 gene, Tc r /Ap s This study Xc11bla::Tc(pRKSE) Xc11 bla XCC-1 mutant complemented with cloned bla XCC-1 gene, Tc r /Ap r /Km r Weng et al. ( 29 ) Xc11RM(pFJ-ampR1) Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tc r /Ap r /Km r This study X. campestris pv. begoniae 59 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. dieffenbachiae 65 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. mangiferaeindcae 38 Wild-type strain isolated in Taiwan, Ap s Tseng lab collection X. campestris pv. phaseoli 73 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. citri 60 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. glycines 69 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. oryzae pv. oryzae 21 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. vesicatoria Xv64 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xv3240 European strain, Ap s Ojanen et al. ( 20 ) Serratia marcescens 6 Wild-type strain isolated in Taiwan, Ap r L. T. Wu, China Medical University Open in a separate window a Yi-Hsiung Tseng's laboratory collection.

Techniques: Plasmid Preparation, Isolation, Mutagenesis, Construct, Clone Assay

Journal:

Article Title: Constitutive Expression of a Chromosomal Class A (BJM Group 2) ?-Lactamase in Xanthomonas campestris

doi: 10.1128/AAC.48.1.209-215.2004

Figure Lengend Snippet: Inserts of the plasmids carrying blaXCC-1 and/or ampR1 from X. campestris pv. campestris strain 11. The 4.0-kb Sau3A1 fragment in pCH1, the 2.5-kb StuI-XbaI fragment in pFY13-9BSRX, the 1.7-kb EcoRI-SstI fragment in pBKE, the 0.5-kb PstI-SstI fragment in pBKX, the 1.0-kb SmaI-SstI fragment in pOKSS, the 1.3-kb PstI-XbaI fragment in pFJ-ampR1, the 0.5-kb PstI-PvuII fragment in pBX, and the same fragment in the opposite orientation in pRX are shown. The underlined restriction sites are from the multiple cloning sites of the vectors.

Article Snippet: Antibiotics were added when necessary: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Characteristic(s) Source or reference E. coli DH5α endA1 hsdR17 (r k − m k + ) supE44 thi-1 recA gyrA relA1 φ80d lacZ ΔM15 Δ( lacZYA - argF ) Bethesda Research Laboratories ATCC 25922 Reference strain for MIC tests American Type Culture Collection E. coli (pFY13-9BSRX) DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and bla XCC-1 genes, Km r /Ap s This study E. coli (pBX) DH5α with plasmid pBX carrying the bla XCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tc r /Ap s This study E. coli (pRX) DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tc r /Ap s This study X. campestris pv. campestris Xc2 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection a Xc6 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc85 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc17 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11A Spontaneous avirulent mutant from Xc11, Ap r Yang and Tseng ( 33 ) Xc11bla::Tc Xc11 bla XCC-1 mutant constructed by inserting a Tc r cartridge into the Sma I site of the bla XCC-1 gene, Tc r /Ap s Weng et al. ( 29 ) Xc11RM Xc11 ampR1 mutant constructed by inserting a Tc r cartridge into the Sal I site of the ampR1 gene, Tc r /Ap s This study Xc11bla::Tc(pRKSE) Xc11 bla XCC-1 mutant complemented with cloned bla XCC-1 gene, Tc r /Ap r /Km r Weng et al. ( 29 ) Xc11RM(pFJ-ampR1) Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tc r /Ap r /Km r This study X. campestris pv. begoniae 59 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. dieffenbachiae 65 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. mangiferaeindcae 38 Wild-type strain isolated in Taiwan, Ap s Tseng lab collection X. campestris pv. phaseoli 73 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. citri 60 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. glycines 69 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. oryzae pv. oryzae 21 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. vesicatoria Xv64 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xv3240 European strain, Ap s Ojanen et al. ( 20 ) Serratia marcescens 6 Wild-type strain isolated in Taiwan, Ap r L. T. Wu, China Medical University Open in a separate window a Yi-Hsiung Tseng's laboratory collection.

Techniques: Clone Assay

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Inhibition zones of different A. altissima extracts against pathogenic bacteria.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Inhibition, Bacteria, Concentration Assay

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Antibacterial activity of A. altissima methanolic extract expressed as MIC and MBC values.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Activity Assay

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Percentages of biofilm eradication after treatment with various concentrations of A. altissima extracts.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Release of cell content of S. aureus ATCC 25923 and E.coli ATCC 25922 evaluated by measuring and quantifying the absorbance at 260 nm ( A ) and 280 nm ( B ) treated with methanolic extracts of A. altissima from different regions. Control (CTR): bacteria were untreated with plant extracts. Results are expressed as mean ± SD. Absorbance values are expressed as mean ± SD. Comparison of absorbance between CTR and E. coli or S. aureus treated with leaf extracts from each studied region were performed using the unpaired two-tailed Student’s t -test. Significant differences among tested groups are indicated by different letters. a p < 0.001 E. coli values significantly different from CTR and b p < 0.001 S. aureus values significantly different from CTR.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Control, Bacteria, Comparison, Two Tailed Test

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: ROS production by E. coli and S. aureus strains after treatment with the leaf extracts of A. altissima from different regions. Control (CTR): bacteria were untreated with plant extracts. Results are expressed as mean ± SD. Comparisons of fluorescence intensity between CTR and E. coli or S. aureus treated with leaf extracts from each studied region were performed using the unpaired two-tailed Student’s t -test. Significant differences among tested groups are indicated by different letters. a p < 0.001 indicates E. coli values are significantly different from CTR and b p < 0.001 indicates S. aureus values are significantly different from CTR.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Control, Bacteria, Fluorescence, Two Tailed Test

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Quantification of MDA production by E. coli ATCC 25922 and S. aureus ATCC 25923 after 24 h of treatment with extracts of A. altissima from different studied regions. Control (CTR): bacteria were untreated with plant extracts. MDA levels are expressed as mean ± SD. Comparison of MDA rates between CTR and E. coli or S. aureus treated with leaf extracts from each studied region were performed using the unpaired two-tailed Student’s t -test. ** p < 0.01 and *** p < 0.001 significantly different from CTR.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Control, Bacteria, Comparison, Two Tailed Test

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Determination of SOD activity (SOD/mg protein) in E. coli and S. aureus in the presence of A. altissima extracts. Control (CTR): bacteria were untreated with plant extracts. Data reported as mean ± SD. SOD activity comparison between CTR and E. coli or S. aureus treated with leaves extracts from each studied region were performed using the unpaired two-tailed Student’s t -test. *** p < 0.001 significantly different from CTR.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Activity Assay, Control, Bacteria, Comparison, Two Tailed Test

Journal: Antibiotics

Article Title: Chemical Composition of Ailanthus altissima (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction

doi: 10.3390/antibiotics12081253

Figure Lengend Snippet: Determination of CAT activity (U/mg protein) in E. coli and S. aureus in the presence of A. altissima extracts. Control (CTR): bacteria were untreated with plant extracts. Data reported as mean ± SD. CAT activity comparison between CTR and E. coli or S. aureus treated with leaf extracts from each studied region were performed using the unpaired two-tailed Student’s t -test. *** p < 0.001 significantly different from CTR.

Article Snippet: In E. coli ATCC 25922, there was a decrease of 2 to 98 times compared to the control group with the lowest value observed in the samples from Sousse and Tlemcen.

Techniques: Activity Assay, Control, Bacteria, Comparison, Two Tailed Test